Multi-Scale Linkages between Single-Molecule Integrin Dynamics and Cell Protrusion

Probing single molecule dynamics.

The room temperature dynamics of single sulforhodamine 101 molecules dispersed on a glass surface are investigated on two different time scales with near-field optics. On the 10(-2)- to 10(2)-second time scale, intensity fluctuations in the emission from single molecules are examined with polarization measurements, providing insight into their spectroscopic properties. On the nanosecond time sc...

Single-molecule enzymatic dynamics.

Enzymatic turnovers of single cholesterol oxidase molecules were observed in real time by monitoring the emission from the enzyme's fluorescent active site, flavin adenine dinucleotide (FAD). Statistical analyses of single-molecule trajectories revealed a significant and slow fluctuation in the rate of cholesterol oxidation by FAD. The static disorder and dynamic disorder of reaction rates, whi...

Linkages between large-scale climate patterns and the dynamics of Arctic caribou populations

K. Joly ([email protected]), National Park Service, Gates of the Arctic National Park and Preserve, and Arctic Network Inventory and Monitoring Program, Fairbanks, AK 99709, USA. D. R. Klein and F. S. Chapin III, Univ. of Alaska Fairbanks, Inst. of Arctic Biology, Fairbanks, AK 99775, USA. D. L. Verbyla and T. S. Rupp, Univ. of Alaska Fairbanks, School of Natural Resources and Agricultural Scie...

Single Molecule Dynamics at Surfaces

Changes in single-molecule integrin dynamics linked to local cellular behavior

Recent advances in light microscopy permit visualization of the behavior of individual molecules within dense macromolecular ensembles in live cells. It is now conceptually possible to relate the dynamic organization of molecular machinery to cellular function. However, inherent heterogeneities, as well as disparities between spatial and temporal scales, pose substantial challenges in deriving ...